Composite

Part:BBa_K3168007:Design

Designed by: Eva Hanckmann, Harm van der Veer, Claire Michielsen   Group: iGEM19_TU_Eindhoven   (2019-09-17)


dCas9-BRET


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2143
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 261
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A strep tag is included at the end for protein purification. The two proteins are connected by a (GGS)2.

Source

Basic part (BBa_K3168001) and (BBa_K3168006).

References